Composite

Part:BBa_K2810011

Designed by: Ryan Coates   Group: iGEM18_Cardiff_Wales   (2018-09-26)


mCherry under control of the bacterial promoter and terminator

mCherry, codon optimised for Nicotiana benthamiana, under control of part BBa_J23100 (bacterial promoter) and BBa_B0015 (bacterial terminator). The RBS is part BBa_B0030 between the promoter and CDS. This part was used to show that the coding sequence of our mCherry part (BBa_K2810009) was fully functional. The plasmid it is in (pSB1C3) encodes a chloramphenicol resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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